Overview
- Detection kits for PLMVd
- Available in 1 format for analysis:
Peach latent mosaic viroid (PLMVd) is a viroid specific to peach and consisting of a circular single-stranded RNA of 336-339 nt. It has been widely distributed around the world transmitted by graft propagation, and to a lesser extent by aphids. Although most infections occur without conspicuous symptoms, certain PLMVd isolates induce leaf mosaics, blotches and in the most extreme cases albinism (peach calico, PC), flower streaking, delays in foliation, flowering and ripening, deformations and decolorations of fruits, bud necrosis, stem pitting and premature ageing of the trees, which also adopt a characteristic growing pattern (open habit). PLMVd can be detected by molecular methods including polyacrylamide gel electrophoresis, dot-blot hybridization with radioactive and non-radioactive probes and RT-PCR.
Click to expand options
- Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
- Specific Primer and Probe mix for the pathogen/virus/viroid of interest
- Primer and Probe mix
- Positive and negative control to confirm the integrity of the kit reagents
- Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
- Nuclease-free water
- Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix
For research use only and NOT intended for in vitro diagnostics.
Details
Supporting Data
Storage Conditions
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival.
Component | Cat. TM64250 (100 preps) | Cat. TM64210 (100 preps) |
---|---|---|
MDx TaqMan 2X RT-PCR Master Mix | 2 x 700 μL | - |
PLMVd Primer & Probe Mix | 280 μL | 280 μL |
PLMVd Positive Control | 150 μL | 150 μL |
Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
Product Insert | 1 | 1 |