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Pure RNA, Pure Results.
Exploring the power of RNA
Why Our Powerful and Highly-Sensitive RNA Extraction Technology is Best-in-Class
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No RNA Carriers Required
RNA carriers negatively impact the efficacy of downstream applications like NGS. Our RNA extraction technology is highly sensitive & linear down to a few cells without the need for carrier RNA.
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No Hazardous Compounds
Carryover of hazardous compounds such as Phenol/Chloroform can negatively impact your downstream results. Unlike other common extraction methods, our RNA extraction technology does not require the use of such hazardous compounds.
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Size (and Content) Matters
Silica has shown a bias towards sequences that have a high molecular weight and a high GC content. Our RNA extraction technology shows no bias towards GC content or molecular weight, providing a true representation of RNA content.
Trusted by Leading Institutions Worldwide
"We have been using Norgen's RNA kits for many years for our colorectal cancer diagnosis projects and their products proved to be very efficient in RNA extraction. Norgen's products worked perfectly and helped us to engage in new molecular approaches."
Figure 1. High Quality of Isolated RNA with Complete Size Range.
Unlike most competitors' kits, Norgen's Total RNA Purification Kit allows for the isolation of all sizes of RNA, from the very large RNA down to the microRNA, without the use of phenol. Total RNA was isolated from 1 x 109 E. coli cells using Norgen’s Total RNA Purification Kit and a competitor’s kit. Five microliters and 1 µL of the 50 µL isolated RNA was analyzed on an agarose gel (Panel A) and the Agilent® 2100 BioAnalyzer RNA Nano 6000 chip (Panel B), respectively. Note the presence of small RNA species (red square) in the samples isolated via Norgen's kit and the absence of these RNA species in the competitor RNA preparation.
Figure 5. Linear and Sensitive Isolation of Both Large and Small RNA.
Norgen’s Total RNA Purification Kit allows consistent isolation of both large and small RNA from different input amounts. Total RNA was isolated from 10 to 100,000 HeLa cells using Norgen's Total RNA Purification Kit (blue), a competitor’s silica-based kit (green) and a phenol-based RNA extraction method (red). Relative expression of miR-21 (Panel A), and S15 (Panel B) was determined by RT-qPCR of total RNA samples. In brief, one microliter of the 50 µL isolated RNA was then subjected to a 20 µL reverse transcription using miR-21 stem-loop reverse primer or oligo dT primer. Two microliters of the reverse transcription was used in a 20 µL real-time PCR reaction with primers to detect the human miR-21 (Panel A) and the S15transcripts (Panel B). The resulting threshold cycle (Ct) values were plotted against input cell number. RNA isolated using Norgen's Total RNA Purification had the best linearity (higher R2) and sensitivity (lower Ct) for both large RNA (S15) and small RNA (miR-21).
Figure 8. Better Diversity of miRNA Detected from Plasma.
Norgen's Total RNA Purification Kit isolates miRNA from plasma with better diversity than a leading competitor. Total RNA including miRNA was isolated from 100 µL of plasma using Norgen's Total RNA Purification Kit or 625 µL of plasma using Competitor A's leading miRNA Kit, and was applied to an NCode expression profiling kit. Microarray images suggested that Norgen's Total RNA Purification Kit (left) isolates a better diversity of miRNA from smaller input amount of plasma than the competitor’s miRNA kit (right). Image courtesy of LC Sciences, Houston.
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SKU 17200 | Spin Column | 50 Preps
USD283.00
For the rapid purification of total RNA - including microRNA - without the use of phenol
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SKU 37500 | Spin Column | 100 Preps
USD520.00
For the rapid purification of total RNA - including microRNA - without the use of phenol
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SKU 25800 | Spin Column | 50 Preps
USD335.00
For the rapid purification of total RNA (including microRNA) from plants and fungi
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SKU 23600 | Spin Column | 50 Preps
USD277.00
For rapid and efficient clean-up and concentration of total RNA, including microRNA, without phenol
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SKU 75100 | Spin Column | 25 Preps
USD359.00
Rapid purification of microRNA from urine without phenol
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SKU 29000 | Spin Column | 25 Preps
USD287.00
Rapid purification of microRNA from urine without phenol
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SKU 25700 | Spin Column | 50 Preps
USD390.00
For purification of total RNA (including microRNA) from all types of tissues including fiber-rich tissues
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SKU 75000 | 25 Preps
USD320.00
For the rapid purification of microRNA without phenol
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SKU 21300 | 25 Preps
USD256.00
For the rapid purification of microRNA without phenol
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SKU 29600 | Maxi Slurry | Variable
USD561.00
Rapid purification of total RNA from 5 mL - 10 mL urine without phenol
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SKU 25300 | Spin Column | 50 Preps
USD448.00
For the rapid and efficient extraction and purification of RNA (including microRNA) from FFPE samples
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SKU 21200 | Spin Column | 50 Preps
USD349.00
For the rapid extraction and purification of total RNA from leukocytes
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Select Publications
| Title | Combined MEK and JAK/STAT3 pathway inhibition effectively decreases SHH medulloblastoma tumor progression |
| Journal | Communications Biology |
| Authors | Zagozewski, J.; Borlase, S.; Guppy, B.J.; et al. |
| Title | A nuclear function for an oncogenic microRNA as a modulator of snRNA and splicing |
| Journal | Molecular Cancer |
| Authors | El Fatimy, R.; Zhang, Y.; Deforzh, E.; et al. |
| Title | Multiomic analysis of microRNA-mediated regulation reveals a proliferative axis involving miR-10b in fibrolamellar carcinoma |
| Journal | JCI Insight |
| Authors | Francisco, A.; Kanke, M.; Massa, A.; et al. |
| Title | Blood circulating miR-28-5p and let-7d-5p associate with premature ageing in Down syndrome |
| Journal | Science Direct |
| Authors | Morsiani, C.; Bacalini, M.G.; Collura, S.; et al. |
| Title | Contributions of Circulating microRNAs for Early Detection of Lung Cancer |
| Journal | Cancers 2022 |
| Authors | Vykoukal, J.; Fahrmann, J.F.; Patel, N.; et al. |
| Title | Synergistic Analysis of Circulating Tumor Cells Reveals Prognostic Signatures in Pilot Study of Treatment-Naïve Metastatic Pancreatic Cancer Patients |
| Journal | Biomedicines 2022 |
| Authors | Owen, S.; Prantzalos, E.; Gunchick, V.; et al. |
| Title | Validation of Isolation Methodology and Endogenous Control Selection for qRT-PCR Assessment of Microrna Expression in Serum and Urine Exosomes |
| Journal | Blood 2014 |
| Authors | Crossland, R.E.; Norden, J.; Bibby, L.; et al. |
| Title | Evaluation of different RNA extraction methods for high-quality total RNA and mRNA from Erwinia amylovora in planta |
| Journal | European Journal of Plant Pathology 2016 |
| Authors | Kałużna, M.; Kuras, A.; Mikiciński, A.; Puławska, J. |
| Title | Comparison of Whole Blood RNA Preservation Tubes and Novel Generation RNA Extraction Kits for Analysis of mRNA and MiRNA Profiles |
| Journal | PLOS ONE 2014 |
| Authors | Häntzsch, M.; Tolios, A.; Beutner, F.; Nagel, D.; Thiery, J.; et al. |
| Title | Simultaneous extraction of mRNA and microRNA from whole blood stabilized in tempus tubes |
| Journal | BMC Res Notes 2019 |
| Authors | Richards, J.; Unger, E.R.; Rajeevan, M.S. |
| Title | Total RNA extraction from tissues for microRNA and target gene expression analysis: not all kits are created equal |
| Journal | BMC Biotechnology 2018 |
| Authors | Brown, R.A.M.; Epis, M.R.; Horsham, J.L.; et al. |