High Quality of Isolated RNA and Complete Size Range
Figure 1. High Quality of Isolated RNA with Complete Size Range. Unlike most competitors' kits, Norgen's Total RNA Purification Kit allows for the isolation of all sizes of RNA, from the very large RNA down to the microRNA, without the use of phenol. Total RNA was isolated from 1 x 109 E. coli cells using Norgens Total RNA Purification Kit and a competitors kit. Five microliters and 1 μL of the 50 μL isolated RNA was analyzed on an agarose gel (Panel A) and the Agilent® 2100 BioAnalyzer RNA Nano 6000 chip (Panel B), respectively. Note the presence of small RNA species (red square) in the samples isolated via Norgen's kit and the absence of these RNA species in the competitor RNA preparation.
Superior Diversity of miRNA Detected from Plasma
Figure 2. Superior Diversity of miRNA Detected from Plasma. Norgen's Total RNA Purification Kit isolates miRNA from plasma with better diversity than a leading competitor. Total RNA including miRNA was isolated from 100 μL of plasma using Norgen's Total RNA Purification Kit or 625 μL of plasma using Competitor A's leading miRNA Kit, and was applied to an NCode expression profiling kit. Microarray images suggested that Norgen's Total RNA Purification Kit (left) isolates a better diversity of miRNA from smaller input amount of plasma than the competitors miRNA kit (right). Image courtesy of LC Sciences, Houston.
Linear and Sensitive Isolation of Both Large and Small RNA
Figure 3. Linear and Sensitive Isolation of Both Large and Small RNA. Norgens Total RNA Purification Kit allows consistent isolation of both large and small RNA from different input amounts. Total RNA was isolated from 10 to 100,000 HeLa cells using Norgen's Total RNA Purification Kit (blue), a competitors silica-based kit (green) and a phenol-based RNA extraction method (red). Relative expression of miR-21 (Panel A), and S15 (Panel B) was determined by RT-qPCR of total RNA samples. In brief, one microliter of the 50 μL isolated RNA was then subjected to a 20 μL reverse transcription using miR-21 stem-loop reverse primer or oligo dT primer. Two microliters of the reverse transcription was used in a 20 μL real-time PCR reaction with primers to detect the human miR-21 (Panel A) and the S15transcripts (Panel B). The resulting threshold cycle (Ct) values were plotted against input cell number. RNA isolated using Norgen's Total RNA Purification had the best linearity (higher R2) and sensitivity (lower Ct) for both large RNA (S15) and small RNA (miR-21).
