Overview

Botrytis cinerea is a ubiquitous fungal pathogen, often present as a latent infection, which can in some cases later develops into damaging symptomatic infections in a wide range of plant species including economically important crops such as vegetables, ornamentals, bulbs and fruits. Consequently, an apparently healthy plant can deteriorate suddenly due to the development of latent or quiescent infection into visible disease. Sensitive and reliable methods to monitor the fungus in plant tissue are essential to enable the study of the factors affecting the progression from latent to aggressive disease, and to allow for early detection and diagnosis of the disease.

For research use only and NOT intended for in vitro diagnostics.

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Supporting Data

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TaqMan PCR Kit - Figure 1. Example of TaqMan PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR.

TaqMan PCR Kit - Figure 2. Example of TaqMan PCR Negative result. No target DNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR..

TaqMan PCR Kit - Figure 3. Example of TaqMan PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for DNA purification.

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