Erwinia amylovora TaqMan PCR Detection Kits
For research use only and NOT intended for in vitro diagnostics.
Lyophilized
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Overview
- Detection kits for the Erwinia amylovora
- Available in TaqMan format for analysis
Erwinia amylovora is a Gram negative, rod-shaped bacterium belonging to the family Enterobacteriaceae. It was the first bacterium identified as a plant pathogen and was shown to be the causative agent of the necrotic disease fireblight. The most economically important hosts are apple and pear, however infection can occur in a range of other plants including Cotoneaster, Cratagus and Pyracantha. During severe disease outbreaks, the rapid spread of bacteria through host tissue can lead to the loss of entire trees in a single growing season. E. amylovora infection of blossom can greatly reduce crop yield and marketability. Fireblight is therefore a highly destructive disease and is of major economic concern to fruit growers world-wide.
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- Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
- Specific Primer and Probe mix for the pathogen/virus/viroid of interest
- Primer and Probe mix
- Positive and negative control to confirm the integrity of the kit reagents
- Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
- Nuclease-free water
- Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix
For research use only and NOT intended for in vitro diagnostics.
Details
Supporting Data
Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Component | Cat. TM35150 (100 preps) | Cat. TM35110 (100 preps) |
---|---|---|
MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | - |
Erwinia amylovora Primer & Probe Mix | 280 μL | 280 μL |
Erwinia amylovora Positive Control | 150 μL | 150 μL |
Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
Product Insert | 1 | 1 |