Hop Stunt Viroid (HSVd) TaqMan RT-PCR Detection Kits
For research use only and NOT intended for in vitro diagnostics.
Overview
- Detection kits for HSVd
- Available in TaqMan format for analysis
The Hop Stunt Viroid (HSVd) is one of a group of the smallest self-replicating RNAs pathogenic to plants. The viroid is a covalently closed, circular, single-stranded RNA consisting of 297 nucleotides. Strains of HSVd can be found to infect citrus, grapes, stone fruit and cucumber, and have been detected in Europe, Asia, North America, Australia and the Middle East. To prevent or reduce the negative effects caused by the viroid it is necessary to use viroid-free planting material. Methods for the identification of this viroid in host plants should help to control the spread of this agriculturally important plant pathogen.
Click to expand options
- Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
- Specific Primer and Probe mix for the pathogen/virus/viroid of interest
- Primer and Probe mix
- Positive and negative control to confirm the integrity of the kit reagents
- Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
- Nuclease-free water
- Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix
For research use only and NOT intended for in vitro diagnostics.
Details
Supporting Data
Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Component | Cat. TM38950 (100 preps) | Cat. TM38910 (100 preps) |
---|---|---|
MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | - |
HSVd Primer & Probe Mix | 280 μL | 280 μL |
HSVd Positive Control | 150 μL | 150 μL |
Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
Product Insert | 1 | 1 |