Mycoplasma TaqMan PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Mycoplasma TaqMan PCR Detection Kits SMART Save USD$699.00 USD$559.20 SKU TM33150 Format PCR Probe/Primer and Control Set Size 100 Reactions Quantity Overview Details Documentation Citations Overview Detection kits for Mycoplasma DNA Available in TaqMan format for analysis Mycoplasma is a common and serious contaminant of cell cultures. Mycoplasma is often not visible and does not respond to antibiotics, and therefore it is a major issue that requires monitoring and early detection. Up to 30% of cell cultures may be contaminated with Mycoplasmas, the main contaminants being the species M. orale, M. laidlawii, M. arginini and M. hyorhinis. These organisms produce a variety of effects on the infected cells in culture, including changes in metabolism growth, viability and morphology, thereby altering the phenotypic characteristics of the host cells. Therefore there is an absolute requirement for routine, periodic assays to diagnose possible Mycoplasma contamination of all cell cultures, particularly continuous or established cell lines. TaqMan PCR Kit Click to expand options Mycoplasma TaqMan PCR Kit, 100 reactions Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality Specific Primer and Probe mix for the pathogen/virus/viroid of interest Primer and Probe mix Positive and negative control to confirm the integrity of the kit reagents Mycoplasma TaqMan PCR Probe/Primer Set and Controls, 100 reactions Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest Nuclease-free water Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix For research use only and NOT intended for in vitro diagnostics. Details Supporting Data Previous TaqMan PCR Kit - Figure 1. Example of TaqMan PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR. TaqMan PCR Kit - Figure 2. Example of TaqMan PCR Negative result. No target DNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR. TaqMan PCR Kit - Figure 3. Example of TaqMan PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for DNA purification. Next Click for expanded view Storage Conditions and Product Stability All kit components can be stored for 2 years after the date of production without showing any reduction in performance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. Component Cat. TM33150 (100 preps) Cat. TM33110 (100 preps) MDx TaqMan 2X PCR Master Mix 2 x 700 μL - Mycoplasma Primer & Probe Mix 280 μL 280 μL Mycoplasma Positive Control 150 μL 150 μL Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL Product Insert 1 1 Documentation PROTOCOLS (TM33150) Mycoplasma TaqMan PCR Kit - Protocol (100 rxns) (TM33110) Mycoplasma TaqMan Probe/Primer and Control Set - Protocol (100 rxns) Related Products Mycoplasma TaqMan Probe/Primer and Control Set Fungi/Yeast Genomic DNA Isolation Kit
Mycoplasma TaqMan PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Mycoplasma TaqMan PCR Detection Kits SMART Save USD$699.00 USD$559.20 SKU TM33150 Format PCR Probe/Primer and Control Set Size 100 Reactions Quantity