Plant/Fungi DNA Isolation Kits
For rapid isolation of total DNA from plants and fungi
For research use only and NOT intended for in vitro diagnostics.
Plant/Fungi DNA Isolation Kits
For rapid isolation of total DNA from plants and fungi
Register today to receive an exclusive 15% off* on your first order.
Features and Benefits
- Rapid and simple procedure
- Excellent quality and yield of DNA
- Process a broad spectrum of plant species and filamentous fungi
- Isolate total DNA including pathogen DNA without phenol
- Available in spin column format and 96-well format for high throughput applications
These kits provide a rapid method for the isolation and purification of total DNA from a wide range of plant and fungal species. Total DNA, including genomic DNA, mitochondrial DNA and chloroplast DNA can be purified from fresh or frozen plant tissues, plant cells or fungi samples using this kit. Purified DNA samples can be used for the detection of viral pathogens, as viral DNA is isolated with the plant/fungi DNA. The purified DNA is of the highest integrity, and can be used in a number of downstream applications including PCR, qPCR, SNP, Southern blotting and sequencing.
Plant/Fungi DNA Isolation Kit (Spin Column)
Complete 10 purifications in 45 minutes. This kit offers a maximum loading volume of 650 μL per column, and a maximum binding capacity of 50 μg per column.
Plant/Fungi DNA Isolation 96-Well Kit (High Throughput)
For high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system. Complete 96 purifications in 50 minutes. This kit offers a maximum loading volume of 500 μL per well, and a maximum binding capacity of 50 μg per well.
Plant/Fungi DNA Isolation Kit (Magnetic Bead System)
The DNA is bound to the surface of the magnetic beads under optimized buffer conditions and released using a low salt buffer system. The Plant DNA Isolation Kit (Magnetic Bead System) can be easily adapted to automated magnetic bead separation instruments and work stations.
Plant/Fungi DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)
For high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.
Details
Supporting Data
Kit Specifications
|
|
Maximum Plant Input
|
50 mg
|
Type of Plants Processed
|
All types of plant species and tissue
|
Average Yield from 50 mg of Plant*
|
3-10 μg
|
Average Purity (OD260/280) |
1.8 - 2.0
|
Time to Complete 96 Purifications |
Less than 60 minutes
|
* Average DNA yield will vary depending on the donor
Storage Conditions and Product Stability
Store RNase A at -20°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
Component | Cat. 26200 (50 preps) | Cat. 26250 (250 preps) | Cat. 26900 (192 preps) | Cat. 58200 (50 preps) | Cat. 62400 (192 preps) |
---|---|---|---|---|---|
Lysis Buffer L | 30 mL | 1 x 30 mL 2 x 60 mL |
2 x 60 mL | 60 mL | 2 x 60 mL |
Binding Buffer I | 7 mL | 1 x 7 mL 1 x 25 mL |
25 mL | 7 mL | 25 mL |
Solution WN | 18 mL | 1 x 18 mL 1 x 55 mL |
55 mL | 18 mL | 55 mL |
Wash Solution A | 38 mL | 2 x 38 mL | 2 x 38 mL | - | - |
Elution Buffer B | 15 mL | 30 mL | 30 mL | 8 mL | 30 mL |
RNAse A | 1 vial (80 μL) | 5 vials (5 x 80 μL) | 1 vial | 1 vial | 1 vial |
Magnetic Bead Suspension | - | - | - | 4 x 1.1 mL | 2 x 8.5 mL |
Filter Columns | 50 | 250 | - | - | - |
Spin Columns | 50 | 250 | - | - | - |
Collection Tubes | 100 | 500 | - | - | - |
96-Well Plate | - | - | 2 | - | 2 |
96-Well Collection Plate | - | - | 2 | - | - |
Adhesive Tape | - | - | 4 | - | 2 |
Elution Tubes (1.7 mL) | 50 | 250 | - | 50 | - |
96-Well Elution Plate | - | - | 2 | - | 2 |
Product Insert | 1 | 1 | 1 | 1 | 1 |
Documentation
(26250) Plant/Fungi DNA Isolation Kit (Spin Column) - Protocol (250 Preps)
(26900) Plant/Fungi DNA Isolation 96 Well Kit (High Throughput) - Protocol (96-well)
(58200) Plant DNA Isolation Kit (Magnetic Bead System) - Protocol (50 preps)
(62400) Plant DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System) - Protocol (2 x 96-well)
26250 - Plant_Fungi DNA Isolation Kit (250 Prep) - SDS
26900 - Plant_Fungi DNA Isolation Kit (96-Well Plate) - SDS
58200 - Plant DNA Isolation (Magnetic Bead System - 50 Prep) - SDS
62400 - Plant DNA Isolation (Magnetic Bead System - 2x96 Well Plates) - SDS
A Novel Technology to Extract Nucleic Acids from Different Environmental Samples including Plants, Water and Soil
Compatibility of DNA and RNA Extraction Methods for Challenging Plant Species
FAQs
Spin Column, High Throughput, Magnetic Bead System, High Throughput Magnetic Bead System
Poor DNA recovery could be due to one or more of the following:
- Columns/wells have become clogged (Spin column or 96-well plate format).
Do not exceed the recommended amounts of starting materials. The amount of starting material may need to be decreased if the column shows clogging below the recommended levels. See also “Clogged Column” below.
- An alternative elution buffer was used.
It is recommended that the Elution Buffer B supplied with this kit be used for maximum DNA recovery.
- Ethanol was not added to the lysate.
Ensure that the indicated amount of ethanol (70% or 96-100% as mentioned in the protocol) is added to the lysate before binding to the columns/wells or introduction of magnetic bead suspension.
- Ethanol was not added to the Wash Solution.
Ensure the indicated 96-100% ethanol amount is added to the supplied Solution WN and Wash Solution A prior to use.
- This kit has 2 columns (clear - filter columns; grey - most likely silica).
Please make sure the columns were not interchanged. If that happens, then all DNA would be lost in the first step.
Column clogging may be due to one or more of the following reasons:
- Maximum amount of tissue exceeds kit specifications.
The optimal input of plant tissue or fungi (wet weight) for each kit has been described under Kit specifications and also in the product insert.
- Too much cell debris in the lysate supernatant.
The lysate should be centrifuged at 20,000 x g (~14,000 RPM) for 5 minutes and the clean supernatant should be used to proceed.
- Centrifuge temperature is too low.
Ensure that the centrifuge remains at room temperature throughout the procedure. Temperatures below 20°C may cause precipitates to form that can cause the columns to clog.
If the DNA does not perform well in downstream applications, it may be due to one or more of the following:
- PCR reaction conditions need to be optimized.
Take steps to optimize the PCR conditions being used, including varying the amount of DNA template, changing the source of Taq polymerase, looking into the primer design, and adjusting the annealing condition.
- Binding Buffer I was not added to the lysate.
Ensure that the Binding Buffer I is added to the lysate and that it is incubated on ice for 5 minutes prior to spinning down the lysate.
- DNA was not washed as directed in the kit manual.
Traces of salt from the binding step may remain in the sample if the column is not washed as directed in the kit manual. Salt may interfere with downstream applications, and thus must be washed from the column/wells/beads.
- Ethanol carryover.
Ensure that the drying step is performed after the wash steps in order to remove traces of ethanol prior to elution. Ethanol is known to interfere with many downstream applications.
RNA can get co-eluted with DNA. Carry out a digestion with RNase A on the elution if the RNA present will interfere with downstream applications. Refer to manufacturer’s instructions regarding amount of enzyme to use, optimal incubation time and temperature.
Yes, the DNA from this kit is good for long-read-sequencing.
Yes, it is possible to isolate DNA from bone tissue using this kit after decalcification of the tissue. Please feel free to contact our Tech support team at support@norgenbiotek.com for help with decalcification protocol.
Citations
Title | Multigene phylogeny, bioactive properties, enzymatic and dye decolorization potential of selected marine fungi from brown algae and sponges of Mauritius |
Citation | HELIYON 2024. |
Authors | Jessica Mélanie Wong Chin, Daneshwar Puchooa, Theeshan Bahorun, Abdulwahed Fahad Alrefaei, Vidushi S. Neergheen, Rajesh Jeewon |
Title | Phylogenomics and plastome evolution of Lithospermeae (Boraginaceae) |
Citation | BMC Plant Biology 2024. |
Authors | Maryam Noroozi, Farrokh Ghahremaninejad, Mehrshid Riahi & James I. Cohen |
Title | PHARMACOGNOSTIC PROFILES AND DNA BARCODING OF THE STEMBARK OF IRVINGIA GABONENSIS (AUBRY-LECOMTE EX O'RORKE) BAIL. (IRVINGIACEAE): A FORENSIC PHARMACOGNOSY DATA |
Citation | International journal of pharmacognosy 2024. |
Authors | Troy Salvia Malgwi, Cletus Anes Ukwubile * and Musa Yusuf Dibal |
Title | Development of a Real-Time PCR Assay for Early Detection of the Eucalyptus Pathogen Quambalaria eucalypti |
Citation | Preprint 2024. |
Authors | Roberto Faedda 1,* and Gabriela B. Silva 2 |
Title | Optimization of loop-mediated isothermal amplification assay for sunflower mildew disease detection |
Citation | Scientific Reports 2024. |
Authors | Oguzhan Yeni, Mutlu Sen, Semra Hasançebi & Neslihan Turgut Kara |
Title | First European records of Puccinia modiolae and P. platyspora, two native South American rust fungi, and new observations on their life cycle and morphology |
Citation | Mycologia 2024. |
Authors | R. Berndt, M. A. G. Otáloraa, M. Angulob, and J. C. Zamorac |
Title | Isolation of a Novel Pythium Species, P. thermoculicivorax, and Trichoderma sp. from Natural Enzootic Mosquito Larval Infections |
Citation | Journal of Fungi 2024. |
Authors | Ross Joseph Ross Joseph ScilitPreprints.orgGoogle Scholar View Publications 1,Constance Darrisaw 2,Aaron Lloyd 2ORCID,David Hoel 2,* andNemat O. Keyhani 1,*ORCID |
Title | High variability of microbial diversity from lampenflora of two bas-reliefs in the Pommery Champagne cellar |
Citation | International Biodeterioration & Biodegradation 2024. |
Authors | Stéphanie Eyssautier-Chuine a, Ludovic Besaury b, Nicolas Richet c, Nathalie Vaillant-Gaveau c, Sébastien Laratte a, Marine Rondeau d, Clément Pierlot d, Alexandre Brunet a, Maxime Gommeaux a |
Title | Identifying Fire Blight-Resistant Malus sieversii Rootstocks Grafted with Cultivar 'Aport' Using Monitoring Data |
Citation | Horticulture 2024. |
Authors | Aisha Taskuzhina, Alexandr Pozharskiy, Zhulduzay Jumanova, Sagi Soltanbekov, Zhanna Issina, Nazym Kerimbek, Anastasiya Kapytina, Marina Khusnitdinova, Abay Sagitov, Alibi Darubayev, Aigerim Seisenova, Yerlan Omarov and Dilyara Gritsenko |
Title | Evaluation of the Hypoglycemic Potential of Endophytic Fungi from Leaves Extract of Moringa Oliefera (Pakistan), its Identification and GC-MS Analysis |
Citation | Global Pharmaceutical Sciences Review 2024. |
Authors | Bushra Rehman a Sumera Afzal Khan b Hira Ikram c Rabiya Nasir d Aiman Umar |