Saccharomyces cerevisiae TaqMan PCR Detection Kits
For research use only and NOT intended for in vitro diagnostics.
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Overview
- Detection kits for Saccharomyces cerevisiae
- Available in TaqMan format for analysis
Saccharomyces cerevisiae plays a beneficial role in wine fermentation, in which it is the predominant species. Despite the beneficial role of S. cerevisiae in the food industry for food and beverage production, it is able to cause spoilage in wines. Uncontrolled yeast growth can alter the chemical composition of wine, detracting from its sensory properties of appearance, aroma, and flavour. If these faults are severe, the wine is rejected by consumers. S. cerevisiae contamination is found mainly in sweet wines, where fermentable sugar can support growth, and also in semidry bottled wines. Therefore a rapid and sensitive method to detect S. cerevisiae contamination is required to prevent wine spoilage.
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- Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
- Specific Primer and Probe mix for the pathogen/virus/viroid of interest
- Primer and Probe mix
- Positive and negative control to confirm the integrity of the kit reagents
- Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
- Nuclease-free water
- Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix
For research use only and NOT intended for in vitro diagnostics.
Details
Supporting Data
Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Component | Cat. TM33350 (100 preps) | Cat. TM33310 (100 preps) |
---|---|---|
MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | - |
Saccharomyces cerevisiae Primer & Probe Mix | 280 μL | 280 μL |
Saccharomyces cerevisiae Positive Control | 150 μL | 150 μL |
Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
Product Insert | 1 | 1 |