Staphylococcus aureus Quantified Bacterial DNA Standard To be used as a positive control or PCR quantification standard for Staphylococcus aureus. For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Staphylococcus aureus Quantified Bacterial DNA Standard To be used as a positive control or PCR quantification standard for Staphylococcus aureus. SMART Save USD$659.00 USD$527.20 SKU 28301 Size 250 µL Quantity Overview Details Documentation Citations Overview Quantified standard to be used as a positive control or PCR quantification standard Vigorously quantified using multiple methods Mastitis is the single most costly disease of dairy cattle resulting in the reduction of milk yield and quality. The inflammation of the utter is mainly caused by bacteria, and Staphylococcus aureus (S. aureus) is often considered the most common cause of contagious mastitis in dairy herds. S. aureus infection is estimated to be present in up to 90% of dairy farms and is responsible for 35% of the economic loss in the dairy industry. S. aureus is a facultatively anaerobic, Gram positive bacterium. The majority of S. aureus strains are catalase-positive and coagulase-positive, which forms the basis of traditional identification methodology. Staphylococcus aureus Quantified Bacterial DNA Standard is prepared from cultured bacteria using Norgen’s sample preparation technology. The purified DNA is quantified vigorously using multiple methods including spectrophotometry, gel densitometry and real-time PCR. It is intended to be used as a positive control or PCR quantification standard for Staphylococcus aureus. Details Supporting Data Previous Figure 1. Accurate Quantity and High Quality Staphylococcus aureus DNA Standard. Two micro-liters of Norgen’s Staphylococcus aureus Quantified Bacterial DNA Standard and various serial dilutions (blue curves) were used in a 20 µL Staphylococcus aureus-specific PCR reaction using Norgen’s 2X PCR Master Mix (#28007), supplemented with SYBR Green I. The real-time PCR reactions were performed on a Bio-Rad CFXReal-Time PCR System. No template controls with water were also performed (yellow curves). Norgen’s Staphylococcus aureus Quantified Bacterial DNA Standard and various serial dilutions showed consistent amplification down to fewer than 40 copies per reaction. Next Click for expanded view Volume Provided 250 µL DNA Quantity 2 x 104 copies per µL Storage Conditions Upon receipt, store Norgen’s Staphylococcus aureus Quantified Bacterial DNA Standard at -20oC or lower. Avoid multiple freeze-thaw cycles. If needed, prepare smaller working aliquots and store at -20oC or lower. Documentation PROTOCOLS (28301) Staphylococcus aureus Quantified Bacterial DNA Standard - Protocol (250 μL) SAFETY DATA SHEETS (28301) Staphylococcus aureus Quantified Bacterial DNA Standard - Safety Data Sheet
Staphylococcus aureus Quantified Bacterial DNA Standard To be used as a positive control or PCR quantification standard for Staphylococcus aureus. For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Staphylococcus aureus Quantified Bacterial DNA Standard To be used as a positive control or PCR quantification standard for Staphylococcus aureus. SMART Save USD$659.00 USD$527.20 SKU 28301 Size 250 µL Quantity