Zika Virus (ZIKV) TaqMan RT-PCR Detection Kit Dx Intended for in vitro diagnostics use.CE marked version is not available for sale in the United States and Canada Powered by Bioz Zika Virus (ZIKV) TaqMan RT-PCR Detection Kit Dx Price USD$349.00 SKU DxTM62200 Format RT-PCR Size 24 Reactions Quantity Overview Details Documentation Citations Overview CE-IVDD marked in accordance with the European Commission Directive 98/79/EC. Research version available Available in TaqmMan format for analysis The Zika Virus (ZIKV) is an emerging mosquito-borne virus that was first identified in Uganda in 1947 in Rhesus monkeys through monitoring of sylvatic yellow fever. During large outbreaks in French Polynesia and Brazil, national health authorities reported potential neurological and auto-immune complications of ZIKV disease. Agencies investigating the Zika outbreaks are finding an increasing body of evidence about the link between ZIKV and microcephaly. Infection with ZIKV may be suspected based on symptoms and recent history (e.g. residence or travel to an area where ZIKV is known to be present). Zika virus diagnosis can only be confirmed by laboratory testing for the presence of ZIKV RNA in the blood or other body fluids, such as urine or saliva. TaqMan PCR Kit Click to expand options ZIKV TaqMan RT-PCR Kit, 24 reactions Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality Specific Primer and Probe mix for the pathogen/virus/viroid of interest Primer and Probe mix Positive and negative control to confirm the integrity of the kit reagents Details Supporting Data Previous TaqMan PCR Kit - Figure 1. Example of TaqMan One-step RT-PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR. TaqMan PCR Kit - Figure 2. Example of TaqMan One-step RT-PCR Negative result. No target RNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR. TaqMan PCR Kit - Figure 3. Example of TaqMan one-step RT-PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for RNA purification. TaqMan PCR Kit - Figure 4. Detection of ZIKV using TaqMan One-step RT-PCR detection system. The FAM channel indicates the successful detection ZIKV at different copy numbers (109 to 102). Next Click for expanded view Storage Conditions The ZIKV TaqMan RT-PCR Kit Dx is shipped on dry ice. The components of the kit should be frozen upon arrival. If one or more of the components is not frozen when the kit is received, or if any of the components have been compromised during shipment, please contact Norgen Biotek for assistance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 3 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. Component Cat. DxTM62200 (24 rxns) MDx TaqMan 2X RT-PCR Master Mix Dx 550 µL ZIKV Primer & Probe Mix Dx 2 x 70 µL ZIKV Positive Control Dx - 200,000 copies/μL 50 µL Nuclease-Free Water 2 x 1.25 mL Product Insert 1 Documentation PROTOCOLS (DxTM62200) ZIKV TaqMan RT-PCR Kit Dx - Protocol (24 rxns) SAFETY DATA SHEETS (DxTM62200) ZIKV TaqMan RT-PCR Kit Dx - Safety Data Sheet Related Products Zika Virus (ZIKV) TaqMan Probe/Primer and Control Set Total RNA Purification Kit
Zika Virus (ZIKV) TaqMan RT-PCR Detection Kit Dx Intended for in vitro diagnostics use.CE marked version is not available for sale in the United States and Canada Powered by Bioz Zika Virus (ZIKV) TaqMan RT-PCR Detection Kit Dx Price USD$349.00 SKU DxTM62200 Format RT-PCR Size 24 Reactions Quantity