On February 11, 2020 the World Health Organization announced an official name for the coronavirus disease, COVID-19.
In December 2019, an outbreak of respiratory illness started in Wuhan City, Hubei Province, China and has now spread throughout the world to many different countries. This respiratory disease was caused by a novel coronavirus and was initially termed “2019 novel coronavirus” or “2019-nCoV”, however in February of 2020 the World Health Organization (WHO) announced that the official name of the disease is COVID-19. The official name of the coronavirus causing COVID-19 is SARS-CoV-2....Continue Reading
For more resources about 2019 Novel Coronavirus (COVID-19), visit the following links:
Introducing Norgen's PCR detection solutions for coronavirus based on
the protocol and RT-PCR panel published by the CDC for the detection of COVID-19.
These assays are manufactured under Norgen’s ISO 13485:2016 quality system and are for research use only.
|1. Specimens Collection & Preservation||Swab Collection and Total Nucleic Acid Preservation System (Cat. 68800)||50 Units||View product page|
|Saliva RNA Collection and Preservation (Cat. RU53800)||50 Devices||View product page|
|2. RNA Isolation||Total RNA Purification Kit (Cat. 17200, 37500)||50 Preps, 100 Preps||View product page|
|3. PCR based detection and
|2019-nCoV TaqMan RT-PCR Kit (Cat. TM67100, TM67120)||50 reactions, 500 reactions||View product page|
|2019-nCoV Primer & Probe Mixes (Cat. TM67101, TM67130)*||50 reactions, 500 reactions||View product page|
|2019-nCoV RT-PCR Positive Control (Cat. PC67102, PC67120)**||50 reactions, 500 reactions (per tube)||View product page|
|2X One-Step RT-PCR Master Mix (Cat. 28113, 28115)||100 reactions, 500 reactions||View product page|
* This kit includes all 3 assays in individual tubes, which are premixed to the working concentrations recommended by the CDC
** The control plasmid contains the CDC 2019-nCoV markers (N1,N2) and RNase P gene which are compatible with the CDC COVID-19 specific primer/probe sets
Norgen Biotek is not an endorsed supplier of the CDC coronavirus RT-PCR panel or positive controls. Norgen is offering these products based on the sequences that were published by the CDC, and these are being offered to our customers for research use only, not for in vitro diagnostic use
In December 2019, an outbreak of respiratory illness started in Wuhan City, Hubei Province, China and has now spread throughout the world to many different countries. This respiratory disease was caused by a novel coronavirus and was initially termed “2019 novel coronavirus” or “2019-nCoV”, however in February of 2020 the World Health Organization (WHO) announced that the official name of the disease is COVID-19. The official name of the coronavirus causing COVID-19 is SARS-CoV-2.
Coronaviruses are a large family of viruses known to infect both animals and humans. SARS-CoV-2 is a new strain of coronavirus infecting humans that had not been previously detected before the outbreak in China in December 2019. While the SARS-CoV-2 is new, many coronaviruses have been known to infect animals and humans for some time. Coronaviruses are known to commonly infect camels, cattle, cats, and bats. In humans, Coronavirus infections can cause various illnesses from the common cold to more severe diseases such as Middle East Respiratory Syndrome (MERS) and Severe Acute Respiratory Syndrome (SARS).
Human infection is more severe when the coronavirus has originated in animals and spread to humans, as is the case with MERS and SARS. SARS-CoV-2 is a betacoronavirus, similar to MERS and SARS, both of which have their origins in bats. The animal source of SARS-CoV-2 has not yet been identified, however Chinese officials have linked many of the early cases to a large seafood and live animal market, suggesting that the initial transmission was the result of animal-to-person spread. However, many of the later detected cases did not report any exposure to animal markets, indicating that the virus is now spreading via human-to-human contact.
Symptoms of infection with SARS-CoV-2 can range from milder symptoms such as a runny nose, sore throat, cough, and fever to more severe symptoms including pneumonia or breathing difficulties. In some cases infection with SARS-CoV-2 has resulted in death. As with other respiratory illnesses, older people and individuals with pre-existing medical conditions (such as diabetes or heart disease) are more vulnerable to becoming severely ill with the virus.
The Chinese authorities posted the full genome of SARS-CoV-2 on the NIH sequencing database GenBank. The CDC reported that the first cases of COVID-19 in the US that were sequenced are similar to the sequence published by China, therefore adding further evidence to the fact that there was a single, recent emergence of this virus from an animal source.
Accurate diagnosis of COVID-19 is through the use of real-time RT PCR (rRT-PCR) assays for the in vitro qualitative detection of SARS-CoV-2 in respiratory specimens and sera. The CDC has published full guidelines on their website for the collection, storage and handling of specimens, nucleic acid extraction, quality control, the rRT-PCT detection assay (including the probes and primers), and interpreting test results https://www.cdc.gov/coronavirus/2019-ncov/lab/rt-pcr-detection-instructions.html.
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