Blood DNA Isolation Kits
For the rapid preparation of high quality DNA from whole blood
For research use only and NOT intended for in vitro diagnostics.
Blood DNA Isolation Kits
For the rapid preparation of high quality DNA from whole blood
Overview
- High yield and high purity DNA ready for any application
- Available in a variety of formats to properly suit your needs
- Compatible with blood collected on a variety of commercially available tubes
These kits allow for the isolation of DNA from the blood of various species, including humans and will recover genomic DNA, mitochondrial DNA, viral DNA and bacterial DNA. The purified DNA is of excellent quality and yield and completely compatible with any downstream application including PCR, qPCR and genotyping.
Blood DNA Isolation Mini Kit
Norgen’s Blood DNA Isolation Mini Kit is designed for the rapid preparation of DNA from up to 200 µL of whole blood using a rapid spin column protocol. Preparation time for a single sample is less than 30 minutes and each kit contains sufficient materials for 50 preparations.
Blood DNA Isolation Midi Kit
Norgen’s Blood DNA Isolation Kit is designed for the rapid spin column preparation of DNA from 0.3 to 2 mL volumes of whole blood. Preparation time for a single sample is less than 30 minutes, and each kit contains sufficient materials for 20 preparations.
Blood DNA Isolation Maxi Kit
This kit is designed for the rapid preparation of DNA from 3 mL up to 10 mL of whole blood. Preparation time for a single sample is less than 30 minutes.
Blood DNA Isolation 96-Well Kit (High Throughput)
This kit provides a rapid method for the high-throughput isolation of DNA from up to 200 µL of whole blood. Fast and easy processing using either a vacuum manifold or centrifugation.
Blood DNA Isolation Kit (Magnetic Bead System)
Norgen's Blood DNA Isolation Kit (Magnetic Bead System) is designed for the rapid preparation of genomic DNA from up to 200 µL of whole blood from various species, including human. Purification is based on magnetic beads as the separation matrix. Norgen’s magnetic beads bind DNA under optimized salt concentrations and releases the bound DNA under low salt and slightly alkali conditions. The genomic DNA is preferentially purified from other cellular proteinaceous components. Typical yields of genomic DNA will vary depending on the cell density of the blood sample. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with downstream applications including real-time PCR, NGS and microarray analysis.
Blood DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)
96-well format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.
Details
Supporting Data
Kit Specifications
|
|
Maximum Blood Input
|
200 μL
|
Column Binding Capacity
|
> 50 μL
|
Average Yield (200 μL of blood) |
2-8 μg*
|
Time to Complete 96 Purifications
|
45 minutes
|
*Yield will vary depending on the type of blood processed
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.
Component | Cat. 46300 (50 preps) | Cat. 46380 (100 preps) | Cat. 51400 (20 preps) | Cat. 31200 (12 preps) | Cat. 46350 (192 preps) | Cat. 59800 (50 preps) | Cat. 62600 (192 preps) |
---|---|---|---|---|---|---|---|
Lysis Buffer B | 20 mL | 2 x 20 mL | 2 x 40 mL | 2 x 110 mL | 2 x 40 mL | 20 mL | 1 x 40 mL 1 x 20 mL |
Solution WN | 18 mL | 2 x 18 mL | 55 mL | 55 mL | 55 mL | 18 mL | 55 mL |
Wash Solution A | 18 mL | 2 x 18 mL | 2 x 38 mL | 2 x 38 mL | 2 x 38 mL | - | - |
Elution Buffer B | 30 mL | 2 x 30 mL | 30 mL | 30 mL | 2 x 30 mL | 15 mL | 1 x 30 mL 1 x 15 mL |
Proteinase K in Storage Buffer | 1.2 mL | 2 x 1.2 mL | 4.4 mL | 8 mL | 4.5 mL | 1.2 mL | 4 mL |
Magnetic Bead Suspension | - | - | - | - | - | 2 x 1.1 mL | 8.5 mL |
Maxi Spin Columns | - | - | - | 12 | - | - | - |
Mini Spin Columns | 50 | 100 | - | - | - | - | - |
Midi Spin Columns | - | - | 20 | - | - | - | - |
96-Well Plate | - | - | - | - | 2 | - | 2 |
Adhesive Tape | - | - | - | - | 4 | - | 2 |
Collection Tubes | 50 | 100 | 20 | 12 | - | - | - |
96-Well Collection Plate | - | - | - | - | 2 | - | - |
Elution Tubes | 50 | 100 | 20 | 12 | - | 50 | - |
96-Well Elution Plate | - | - | - | - | 2 | - | 2 |
Product Insert | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
Documentation
(46300) Blood DNA Isolation Mini Kit - Protocol (50 prep)
(46380) Blood DNA Isolation Mini Kit - Protocol (100 prep)
(46350) Blood DNA Isolation 96-Well Kit (High Throughput) - Protocol (2 x 96-well)
(51400) Blood DNA Isolation Midi Kit - Protocol (20 prep)
(59800) Blood DNA Isolation Kit (Magnetic Bead System) - Protocol (50 prep)
(62600) Blood DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System) - Protocol (2 x 96-well)
Genomic DNA Isolation from 1 µL – 100 µL of Whole Blood using Norgen’s Blood Genomic DNA Isolation Micro Kit
Effect of Elution Volume on DNA Recovery and Quality using Norgen’s Blood Genomic DNA Isolation Micro Kit
Single Elution vs. Re-Elution: Implications on Concentration and Purity using the Blood Genomic DNA Isolation Micro Kit
46380 - Blood DNA Isolation Mini Kit (100 Prep) - SDS
51400 - Blood DNA Isolation Midi Kit - SDS
31200 - Blood DNA Isolation Maxi Kit - SDS
46350 - Blood DNA Isolation 96-Well Kit - SDS
59800 - Blood DNA Isolation Kit (Magnetic Bead - 50 Prep) - SDS
62600 - Blood DNA Isolation Kit (Magnetic Bead - 2x96 Well Plates) - SDS
Citations
Title | Lack of age-related mosaic loss of W chromosome in long-lived birds |
Journal | Biology Letters.2019. |
Authors | Nancy Trujillo, Mónica Martínez-Pacheco, Cecilia Soldatini, Sergio Ancona, Rebecca C. Young, Yuri V. Albores-Barajas, Alberto H. Orta, Cristina Rodríguez, Tamas Székely, Hugh Drummond, Araxi O. Urrutia and Diego Cortez |
Title | Associations of + 138 Ins/del A and + 5665 G/T polymorphisms of endothelin-1 gene with hypertension in Burmese people in Magway, Myanmar |
Journal | Clinical Hypertension.2019. |
Authors | Win Min Oo, Kyaw Thiha & Myat Mon Khine |