Blood DNA Isolation Kits
For the rapid preparation of high quality DNA from whole blood
For research use only and NOT intended for in vitro diagnostics.
Blood DNA Isolation Kits
For the rapid preparation of high quality DNA from whole blood
Register today to receive an exclusive 15% off* on your first order.
Features and Benefits
- High yield and high purity DNA ready for any application
- Available in a variety of formats to properly suit your needs
- Compatible with blood collected on a variety of commercially available tubes
These kits allow for the isolation of DNA from the blood of various species, including humans and will recover genomic DNA, mitochondrial DNA, viral DNA and bacterial DNA. The purified DNA is of excellent quality and yield and completely compatible with any downstream application including PCR, qPCR and genotyping.
Blood DNA Isolation Mini Kit
Norgen’s Blood DNA Isolation Mini Kit is designed for the rapid preparation of DNA from up to 200 µL of whole blood using a rapid spin column protocol. Preparation time for a single sample is less than 30 minutes and each kit contains sufficient materials for 50 preparations.
Blood DNA Isolation Midi Kit
Norgen’s Blood DNA Isolation Kit is designed for the rapid spin column preparation of DNA from 0.3 to 2 mL volumes of whole blood. Preparation time for a single sample is less than 30 minutes, and each kit contains sufficient materials for 20 preparations.
Blood DNA Isolation Maxi Kit
This kit is designed for the rapid preparation of DNA from 3 mL up to 10 mL of whole blood. Preparation time for a single sample is less than 30 minutes.
Blood DNA Isolation 96-Well Kit (High Throughput)
This kit provides a rapid method for the high-throughput isolation of DNA from up to 200 µL of whole blood. Fast and easy processing using either a vacuum manifold or centrifugation.
Blood DNA Isolation Kit (Magnetic Bead System)
Norgen's Blood DNA Isolation Kit (Magnetic Bead System) is designed for the rapid preparation of genomic DNA from up to 200 µL of whole blood from various species, including human. Purification is based on magnetic beads as the separation matrix. Norgen’s magnetic beads bind DNA under optimized salt concentrations and releases the bound DNA under low salt and slightly alkali conditions. The genomic DNA is preferentially purified from other cellular proteinaceous components. Typical yields of genomic DNA will vary depending on the cell density of the blood sample. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with downstream applications including real-time PCR, NGS and microarray analysis.
Blood DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)
96-well format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.
Details
Supporting Data
Kit Specifications
|
|
Maximum Blood Input
|
200 µL
|
Average Yield (200 µL blood)*
|
4-12 µg
|
Average Purity (OD260/280) |
1.8 - 1.9
|
Time to Complete Purifications |
40 minutes (hands-on time Cat. 59800)
50 minutes (hands-on time Cat. 62600) |
* Average DNA yield will vary depending on the donor
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.
Component | Cat. 46300 (50 preps) | Cat. 46380 (100 preps) | Cat. 51400 (20 preps) | Cat. 31200 (12 preps) | Cat. 46350 (192 preps) | Cat. 59800 (50 preps) | Cat. 62600 (192 preps) |
---|---|---|---|---|---|---|---|
Lysis Buffer B | 20 mL | 2 x 20 mL | 2 x 40 mL | 2 x 110 mL | 2 x 40 mL | 20 mL | 1 x 40 mL 1 x 20 mL |
Solution WN | 18 mL | 2 x 18 mL | 55 mL | 55 mL | 55 mL | 18 mL | 55 mL |
Wash Solution A | 18 mL | 2 x 18 mL | 2 x 38 mL | 2 x 38 mL | 2 x 38 mL | - | - |
Elution Buffer B | 30 mL | 2 x 30 mL | 30 mL | 30 mL | 2 x 30 mL | 15 mL | 1 x 30 mL 1 x 15 mL |
Proteinase K in Storage Buffer | 1.2 mL | 2 x 1.2 mL | 4.4 mL | 8 mL | 4.5 mL | 1.2 mL | 4 mL |
Magnetic Bead Suspension | - | - | - | - | - | 2 x 1.1 mL | 8.5 mL |
Maxi Spin Columns | - | - | - | 12 | - | - | - |
Mini Spin Columns | 50 | 100 | - | - | - | - | - |
Midi Spin Columns | - | - | 20 | - | - | - | - |
96-Well Plate | - | - | - | - | 2 | - | 2 |
Adhesive Tape | - | - | - | - | 4 | - | 2 |
Collection Tubes | 50 | 100 | 20 | 12 | - | - | - |
96-Well Collection Plate | - | - | - | - | 2 | - | - |
Elution Tubes | 50 | 100 | 20 | 12 | - | 50 | - |
96-Well Elution Plate | - | - | - | - | 2 | - | 2 |
Product Insert | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
Documentation
(46300) Blood DNA Isolation Mini Kit - Protocol (50 prep)
(46380) Blood DNA Isolation Mini Kit - Protocol (100 prep)
(46350) Blood DNA Isolation 96-Well Kit (High Throughput) - Protocol (2 x 96-well)
(51400) Blood DNA Isolation Midi Kit - Protocol (20 prep)
(59800) Blood DNA Isolation Kit (Magnetic Bead System) - Protocol (50 prep)
(62600) Blood DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System) - Protocol (2 x 96-well)
Genomic DNA Isolation from 1 µL – 100 µL of Whole Blood using Norgen’s Blood Genomic DNA Isolation Micro Kit
Effect of Elution Volume on DNA Recovery and Quality using Norgen’s Blood Genomic DNA Isolation Micro Kit
Single Elution vs. Re-Elution: Implications on Concentration and Purity using the Blood Genomic DNA Isolation Micro Kit
46380 - Blood DNA Isolation Mini Kit (100 Prep) - SDS
51400 - Blood DNA Isolation Midi Kit - SDS
31200 - Blood DNA Isolation Maxi Kit - SDS
46350 - Blood DNA Isolation 96-Well Kit - SDS
59800 - Blood DNA Isolation Kit (Magnetic Bead - 50 Prep) - SDS
62600 - Blood DNA Isolation Kit (Magnetic Bead - 2x96 Well Plates) - SDS
Citations
Title | Epigenetic modifications in the ferroptosis pathway in cord blood cells from newborns of smoking mothers and their influence on fetal growth |
Citation | Reproductive Toxicology 2024. |
Authors | Eva Barrio a, Diego Lerma-Puertas a b, José Javier Jaulín-Pueyo a c, José Ignacio Labarta a c, Ana Gascón-Catalán |
Title | GENE EXPRESSION AND SINGLE NUCLEOTIDE POLYMORPHISM (rs1140713) OF MICRORNA-126 |
Citation | Iraqi Journal of Agricultural Sciences 2024. |
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Title | JAK2, STAT3 Gene Polymorphisms in Turkish Patients with Behçet's Disease |
Citation | Gazi Medical Journal 2024. |
Authors | Darya Farhoomand Aksoy1, Rahime Aksoy2, Sevim Gönen3, Ümit Ölmez1 |
Title | Bisulfite probing reveals DNA structural intricacies |
Citation | Nucleic Acids Research 2023. |
Authors | Andrew T M Bagshaw, Neil J Gemmell |
Title | Detection of Mycobacterium tuberculosis in Peripheral Blood Mononuclear Cells from Patients with Pulmonary Tuberculosis |
Citation | Jundishapur Journal Of Microbiology 2023. |
Authors | Farimah Yarmohammadi, Ali Farhadi, Narges Hassanaghaei, Gholamreza Rafiei Dehbidi, Farzaneh Zarghampoor, Ehsan Farzanfar, Sepide Namdari and Abbas Behzad Behbahani |
Title | Investigating Genetic and Environmental Substrates of the Relationship between Positive Mental Health and Biological Aging—A Study Protocol |
Citation | Brain Sciences 2023. |
Authors | Fransesca Marcon, Miriam Salemi, Cristina D'Ippolito, Angelo Picardi, Virgilia Toccaceli, Lorenza Nistico, Sabrina Alviti, Ester Sinischali, Francesca Salani, Giorgia Maria Varalda, Emanuela Medda and Corrado Fagnani |
Title | Plasma cell-free RNA profiling of Vietnamese Alzheimer's patients reveals a linkage with chronic inflammation and apoptosis: a pilot study |
Citation | Frontiers 2023. |
Authors | Thien Hoang Minh Cao, Anh Phuc Hoang Le, Tai Tien Tran, Vy Kim Huynh, Bao Hoai Pham, Thao Mai Le, Quang Lam Nguyen, Thang Cong Tran. Trang Mai Tong, The Ha Ngoc Than, Tran Tran To Nguyen & Huong Thi Thanh Ha |
Title | A new nucleotide variant G1358A potentially change growth differentiation factor 9 profile that may affect the reproduction performance of Friesian Holstein cattle |
Citation | Asian Pacific Journal of Reproduction 2016. |
Authors | Afifi Inayah1 , Sri Rahayu1 , Nashi Widodo1*, Widya Ayu Prasdini |
Title | Association of JAZF1 and TSPAN8/LGR5 variants in relation to type 2 diabetes mellitus in a Saudi population |
Citation | Diabetology and Metabolic Syndrome 2015. |
Authors | Khalid Khalaf Alharbi1, Imran Ali Khan1*, Rabbani Syed1, Fawiziah Khalaf Alharbi2, Abdul Khader Mohammed3,4, Benjamin Vinodson3 and Nasser M. Al-Daghri3,4 |
Title | Screening for genetic mutations in LDLR gene with familial hypercholesterolemia patients in the Saudi population |
Citation | Acta Biochimica Polonica 2015. |
Authors | Alharbi, K. K., Kashour, T. S., Al-Hussaini, W., Nbaheen, M. S., Hasanato, R. M., Mohamed, S., ... & Khan, I. A. |