FullRanger 100 bp DNA Ladder
Ideal for accurate visual determination in both large and small cloning applications
For research use only and NOT intended for in vitro diagnostics.
FullRanger 100 bp DNA Ladder
Ideal for accurate visual determination in both large and small cloning applications
Overview
- Ready-to-use
- Quantitative
- Highly Stable
- Precise
- Sixteen discrete fragments ranging from 100 bp to 5000 bp
- Higher intensity reference bands at 500 bp and 1000 bp
The Norgen FullRanger 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our FullRanger contains sixteen discrete fragments ranging from 100 bp to 5000 bp with higher intensity reference bands at 500 bp and 1000 bp. This Ladder is ideal for accurate visual determination in both large and small cloning applications.
Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
Details
Supporting Data
FullRanger 100 bp DNA Ladder (Cat# 11800) - 100 loads
• Sixteen discrete bands, ranging from 100 bp to 5000 bp
• Higher intensity reference bands at 500 bp and 1000 bp
Fragment
|
Size (bp)
|
Mass (ng)
|
1
|
5000
|
55
|
2
|
4000
|
44
|
3
|
3000
|
33
|
4
|
2500
|
28
|
5
|
2000
|
22
|
6
|
1500
|
17
|
7
|
1000
|
50
|
8
|
900
|
35
|
9
|
800
|
31
|
10
|
700
|
43
|
11
|
600
|
24
|
12 | 500 |
56 |
13 | 400 |
16 |
14 | 300 |
22 |
15 | 200 |
15 |
16 | 100 |
11 |
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Stable at room temperature. For longer term storage, -20°C is recommended.
Documentation
Citations
Title | Loop-mediated isothermal amplification for rapid molecular detection of Enterocytozoon bieneusi in faecal specimens |
Journal | Journal of Medical Microbiology. 2015. |
Authors | Nasarudin SN, Zainudin NS, Bernadus M, Mohd Nawi A, Hanafiah A, Osman E |
Title | A Systems Based Investigation into Vitamin D and Skeletal Muscle Repair, Regeneration and Hypertrophy |
Journal | American Journal of Physiology. 2015. |
Authors | Daniel J. Owens, Adam P Sharples, Ioanna Polydorou, Nura Alwan, Timothy F Donovan, Jonathan Tang, Robert G Cooper, William D. Fraser, James P. Morton, Claire Stewart, Graeme L. Close |
Title | Epidemiology of methicillin resistant Staphylococcus aureus (MRSA) isolates from Pakistan. |
Journal | African Journal of Microbiology Research. 2013. |
Authors | Arfat Y, Johnson M, Malik S A, Morrissey J A, and Bayliss C D |
Title | Investigating Host Induced Meiosis in a Fungal Plant Pathogen. |
Journal | Meiosis - Molecular Mechanisms and Cytogenetic Diversity, ISBN: 978-953-51-0118-5. 2012. |
Authors | Saville B, Donaldson M, Doyle C. |