John Cunningham Virus (JCV) TaqMan PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz John Cunningham Virus (JCV) TaqMan PCR Detection Kits Price USD$449.00 SKU TM37210 Format PCR Probe/Primer and Control Set Size 100 Reactions Quantity Overview Details Documentation Citations Overview Detection kits for the JCV CE-IVD marked version available for in vitro diagnostic use Available in TaqMan format for analysis The JC virus (JCV) is a type of human polyomavirus and belongs to the family Papovaviridae. JCV is a double-stranded DNA virus, and is genetically similar to BK virus and SV40. The virus is very common in the general population and it is believed that most people acquire JCV in childhood or adolescence. Typically the infection is subclinical and no of consequence in children with healthy immune systems. The initial site of infection may be the tonsils or the gastrointestinal tract, and the virus then remains latent in the gastrointestinal tract. JCV can also infect the tubular epithelial cells in the kidneys, where it continues to reproduce, shedding virus particles in the urine. Also, JCV can cross the blood-brain barrier into the central nervous system. JCV is known to cause the usually fatal progressive multifocal leukoencephalopathy (PML) by destroying oligodendrocytes in the brain in immunodeficient or immunosuppressed individuals. However, it has not been established whether PML is the result of a primary infection with JCV in a person with impaired immunity or whether it follows reactivation of latent virus. JC virus is also the primary cause of nephropathy (kidney disease) in people who have received a kidney transplant and are on immunosuppressive therapy. TaqMan PCR Kit Click to expand options JCV TaqMan PCR Kit, 100 reactions Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality Specific Primer and Probe mix for the pathogen/virus/viroid of interest Primer and Probe mix Positive and negative control to confirm the integrity of the kit reagents JCV TaqMan PCR Probe/Primer Set and Controls, 100 reactions Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest Nuclease-free water Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix Details Supporting Data Previous TaqMan PCR Kit - Figure 1. Example of TaqMan PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR. TaqMan PCR Kit - Figure 2. Example of TaqMan PCR Negative result. No target DNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR. TaqMan PCR Kit - Figure 3. Example of TaqMan PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for DNA purification. Next Click for expanded view Storage Conditions and Product Stability All kit components can be stored for 2 years after the date of production without showing any reduction in performance. All kit components should be stored at -20°C upon arrival. Component Cat. TM37250 (100 preps) Cat. TM37210 (100 preps) MDx TaqMan 2X PCR Master Mix 2 x 700 μL - JCV Primer & Probe Mix 280 μL 280 μL JCV Positive Control 150 μL 150 μL Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL Product Insert 1 1 Documentation PROTOCOLS (TM37250) JCV TaqMan PCR Kit - Protocol (100 rxns) SAFETY DATA SHEETS (TM37250) JCV TaqMan PCR Kit - Safety Data Sheet Related Products John Cunningham Virus (JCV) TaqMan PCR Kit Dx John Cunningham Virus (JCV) TaqMan Probe/Primer and Control Set Plasma/Serum Cell-Free Circulating DNA Purification Micro Kit
John Cunningham Virus (JCV) TaqMan PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz John Cunningham Virus (JCV) TaqMan PCR Detection Kits Price USD$449.00 SKU TM37210 Format PCR Probe/Primer and Control Set Size 100 Reactions Quantity