Norovirus (NoV) TaqMan RT-PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Norovirus (NoV) TaqMan RT-PCR Detection Kits Price USD$749.00 SKU TM41450 Format RT-PCR Probe/Primer and Control Set Size 100 Reactions Quantity Overview Details Documentation Citations Overview Detection kits for NoV CE-IVD marked version available for in vitro diagnostic use Available in TaqMan format for analysis Norovirus is a single-stranded, non-enveloped RNA virus belonging to the Caliciviridae family. Norovirus is considered the major causative agent of non-bacterial gastroenteritis in the world. The main channel of transmittance is via contaminated food or water, as well as human-to-human contact. Most Noroviruses infecting humans belong to genogroup I and II (particularly genotype 4). Norovirus is very stable in the environment and is resistant to some surface disinfectants such as alcohols and detergents. Moreover, it is very difficult to culture Norovirus in vitro, making its identification by standard microbiological assays challenging. TaqMan PCR Kit Click to expand options NoV TaqMan RT-PCR Kit, 100 reactions Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality Specific Primer and Probe mix for the pathogen/virus/viroid of interest Primer and Probe mix Positive and negative control to confirm the integrity of the kit reagents NoV TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest Nuclease-free water Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix Details Supporting Data Previous TaqMan PCR Kit - Figure 1. Example of Norovirus Positive. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR. TaqMan PCR Kit - Figure 2. Example of Norovirus negative. No target RNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR. TaqMan PCR Kit - Figure 3. Example of PCR inhibition. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for RNA purification. Next Click for expanded view Storage Conditions and Product Stability All kit components can be stored for 1 year after the date of production without showing any reduction in performance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots. Component Cat. TM41450 (100 preps) Cat. TM41410 (100 preps) MDx TaqMan 2X PCR Master Mix 2 x 700 μL - NoV Primer & Probe Mix 280 μL 280 μL NoV Positive Control 150 μL 150 μL Nuclease-Free Water (Negative Control) 1.25 mL 1.25 mL Product Insert 1 1 Documentation PROTOCOLS (TM41450) Norovirus (NoV) TaqMan RT-PCR Detection Kit - Protocol (100 reactions) SAFETY DATA SHEETS (TM41450) Norovirus (NoV) TaqMan RT-PCR Detection Kit - Safety Data Sheet Citations Title Use of dual priming oligonucleotide system-based multiplex RT-PCR combined with high performance liquid chromatography assay for simultaneous detection of five enteric viruses associated with acute enteritis Journal Journal of Virological Methods. 2017. Authors Fan WL, Wang ZW, Qin Y, Sun C, Liu ZM, Jiang YP, Qiao XY, Tang LJ, Li YJ, Xu YG. Related Products Norovirus (NoV) TaqMan RT-PCR Kit Dx Human Immunodeficiency Virus (HIV) TaqMan Probe/Primer and Control Set Plasma/Serum RNA Purification Kit
Norovirus (NoV) TaqMan RT-PCR Detection Kits For research use only and NOT intended for in vitro diagnostics. Powered by Bioz Norovirus (NoV) TaqMan RT-PCR Detection Kits Price USD$749.00 SKU TM41450 Format RT-PCR Probe/Primer and Control Set Size 100 Reactions Quantity
