Norovirus (NoV) TaqMan RT-PCR Detection Kits

For research use only and NOT intended for in vitro diagnostics.
Norovirus (NoV) TaqMan RT-PCR Detection Kits
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Features and Benefits
- Detection kits for NoV
- CE-IVD marked version available for in vitro diagnostic use
- Available in TaqMan format for analysis
Norovirus is a single-stranded, non-enveloped RNA virus belonging to the Caliciviridae family. Norovirus is considered the major causative agent of non-bacterial gastroenteritis in the world. The main channel of transmittance is via contaminated food or water, as well as human-to-human contact. Most Noroviruses infecting humans belong to genogroup I and II (particularly genotype 4). Norovirus is very stable in the environment and is resistant to some surface disinfectants such as alcohols and detergents. Moreover, it is very difficult to culture Norovirus in vitro, making its identification by standard microbiological assays challenging.
Click to expand options
- Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
- Specific Primer and Probe mix for the pathogen/virus/viroid of interest
- Primer and Probe mix
- Positive and negative control to confirm the integrity of the kit reagents
- Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
- Nuclease-free water
- Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix
Details
Supporting Data
TaqMan PCR Kit - Figure 1. Example of Norovirus Positive. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR.
TaqMan PCR Kit - Figure 2. Example of Norovirus negative. No target RNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR.
TaqMan PCR Kit - Figure 3. Example of PCR inhibition. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for RNA purification.
Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival.
Component | Cat. TM41450 (100 preps) | Cat. TM41410 (100 preps) |
---|---|---|
MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | - |
NoV Primer & Probe Mix | 280 μL | 280 μL |
NoV Positive Control | 150 μL | 150 μL |
Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
Product Insert | 1 | 1 |
Documentation
Citations
Title | |
Journal | Journal of Virological Methods. 2017. |
Authors | Fan WL, Wang ZW, Qin Y, Sun C, Liu ZM, Jiang YP, Qiao XY, Tang LJ, Li YJ, Xu YG. |