PCR Purification Kit For the rapid purification of amplified DNA products from PCR mixes For the rapid purification of amplified DNA products from PCR mixes Powered by Bioz PCR Purification Kit For the rapid purification of amplified DNA products from PCR mixes SMART Save USD$439.00 USD$351.20 SKU 24800 Format Spin Column High Throughput Size 2 x 96-Well Plates Quantity Overview Details Documentation Citations Overview Purify amplified DNA ranging from 100 bp -15,000 bp in sizeFast and efficient spin column format Available in a 50 prep size and a 250 prep sizeAlso available in 96 well format This kit enables the rapid purification of amplified DNA products from PCR mixes. It is able to effectively remove PCR by-products including primers, dimers, enzymes, unincorporated nucleotides and mineral oil from the desired PCR product. The purified PCR products are fully compatible with restriction enzyme digestion, ligation into vectors, labeling, sequencing and more. This kit can also be used as an alternative to organic extraction and ethanol precipitation to clean up various enzymatic reactions. The kit is also available in a 96-well format for high-throughput PCR purification. Purification with the 96-well plate can be performed using either a vacuum manifold or centrifugation. Details Supporting Data Previous Figure 1. High Yield Purification. The high yield of Norgen's PCR Purification Kit is illustrated by purifying 3.5 µg of a 500 bp PCR-amplified product from a reaction mixture and comparing the recovery with a competitor. The purifications were performed in triplicate. DNA recovery was quantified by running 350 ng (1/10th) of the input and 5 µL (1/10th) of 50 µL DNA elution on a 1X TAE, 1% agarose gel followed by densitometry. The average yield for each kit is shown in Figure 1. Norgen's kit shows a significantly higher recovery than the competitor's kit. Figure 2. Removal of Primer-Dimers. Primer-dimers present in PCR reactions are effectively removed during the purification process with Norgen's PCR Purification Kit. One microgram of a 700 bp PCR fragment, spiked with 250 pmole of primer (Lane 1) was purified using Norgen's kit, with high recovery and no traces of spiked primer present in the elutions (Lanes 2 and 3). Lane 1 contains 400 ng (40%) of the input, and Lanes 2 and 3 contain 20 µL (40%) of the 50 µL elution (duplicate). Lane M is Norgen's MidRanger 1kb DNA Ladder. Eluted DNA was resolved on a 1X TAE, 1% agarose gel. Next Click for expanded view Kit Specifications - 96-well Binding Capacity Per Well 15 μg Maximum Loading Volume Per Well 500 μL Size of DNA Purified 100 - 15,000 bp Average DNA Recovery > 90% Average Primer Reoval > 90% Minimum Elution Volume 50 μL Time to Complete 96 Purifications 20 minutes Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. Component Cat. 14400 (50 preps) Cat. 45700 (250 preps) Cat. 24800 (192 preps) Binding Buffer C 30 mL 5 x 30 mL 3 x 30 mL Wash Solution A 12 mL 2 x 20 mL 2 x 38 mL Elution Buffer B 8 mL 2 x 30 mL 2 x 15 mL Spin Columns 50 250 - Collection Tubes 50 250 - 96-Well Plate - - 2 Adhesive Tape - - 4 96-Well Collection Plate - - 2 Elution Tubes (1.7 mL) 50 250 - 96-Well Elution Plate - - 2 Product Insert 1 1 2 Documentation PROTOCOLS (14400) PCR Purification Kit (Spin Column) - Protocol (50 prep) (45700) PCR Purification Kit (Spin Column) - Protocol (250 prep) (24800) PCR Purification 96-Well Kit (High Throughput) - Protocol (96-well) SAFETY DATA SHEETS (14400, 45700, 24800) PCR Purification Kit - Safety Data Sheet POSTERS The Inhibitors of Polymerase Chain Reaction in a Biological Culture Citations Title Molecular Confirmation, Identification and Influence of Carbon Source for the Production of Xylanase from Penicillium Citrinum Journal Journal of Drug Delivery and Therapeutics. 2015. Authors Naveen M and Siddalingeshwara K G Title Screening and Identification of Ligninolytic Bacteria for the Treatment of Pulp and Paper Mill Effluent Journal Water, Air, & Soil Pollution. 2015. Authors Rajni Hooda, Nishi K. Bhardwaj, Pamela Singh Title AtROS1 overexpression provides evidence for epigenetic regulation of genes encoding enzymes of flavonoid biosynthesis and antioxidant pathways during salt stress in transgenic tobacco Journal Journal of Experimental Biotechnology. 2015. Authors Poonam Bharti, Monika Mahajan, Ajay K. Vishwakarma, Jyoti Bhardwaj and Sudesh Kumar Yadav Title Recent characterization of cowpea aphid-borne mosaic virus (CABMV) in Bahia State, Brazil, suggests potential regional isolation Journal African Journal of Biotechnology. 2015. Authors Jose Romario Fernandes de Melo, Antonia dos Reis Figueira, Charles Neris Moreira, Antonio Carlos de Oliveira. Title Isolation of Bacteria from Soil sample for ExoPolysaccharide production Journal International Journal of ChemTech Research. 2014. Authors A. Gowsalya, V. Ponnusami and K.R. Sugumaran. Title Isolation and Molecular Identification of Extreme an Halotolerant Staphylococcus Sp. 13cc from Brackish Water of Chilika Lake. Journal Biosciences Biotechnology Research Asia. 2014. Authors P.D. Mahapatra and B.B. Mishra. Title Simultaneous cellulose hydrolysis and bio-electricity generation in a mediatorless Microbial Fuel Cell using a Bacillus flexus strain isolated from wastewater. Journal Research In Biotechnology. 2014. Authors Shankar N, Panchapakesan A, Bhandari S, Ravishankar H. Title TAS2R38 single nucleotide polymorphisms are associated with PROP- but not thermal tasting: a pilot study. Journal Chemosensory Perception. 2013. Authors Bering A, Pickering G, Liang P. Title ”First Generation” Automated DNA Sequencing Technology. Journal Automated DNA Sequencing Technology. Current Protocols in Molecular Biology. 2011. Authors Slatko B, Kieleczawa J, Ju J, Gardner A, Hendrickson C, and Ausubel F. Related Products DNA Gel Extraction Kit Sequencing Reaction Clean-Up Kit
PCR Purification Kit For the rapid purification of amplified DNA products from PCR mixes For the rapid purification of amplified DNA products from PCR mixes Powered by Bioz PCR Purification Kit For the rapid purification of amplified DNA products from PCR mixes SMART Save USD$439.00 USD$351.20 SKU 24800 Format Spin Column High Throughput Size 2 x 96-Well Plates Quantity