Plasmid DNA Kits
For rapid and convenient plasmid DNA preparations
For research use only and NOT intended for in vitro diagnostics.
Overview
- Isolate high quality, high yield plasmid DNA
- Plasmid DNA is ready for various downstream applications including restriction digestion, bacterial transformation, sequencing and more
- Available in 4 formats: MiniPrep, MiniPrep (Magnetic Bead System), 96-Well MiniPrep (Magnetic Bead System), and MaxiPrep
These kits are designed for the rapid preparation of plasmid DNA from Escherichia coli.
Plasmid MiniPrep Kit
This kit is designed for the rapid preparation of plasmid DNA from small cultures of Escherichia coli using convenient spin columns. The plasmid DNA is preferentially purified from other cellular components such as genomic DNA and RNA. This kit is able to purify plasmids up to 13,000 bp in size, and the typical purification yield is up to 20 μg from 1.5 mL of bacterial culture. Purified DNA is of excellent quality for transformation, restriction enzyme digestion, sequencing and more. Also available in a 96-well format.
Plasmid MiniPrep Kit (Magnetic Bead System and High Throughput Magnetic Bead System)
Norgen's Plasmid MiniPrep Kit (Magnetic Bead System) is designed for the rapid preparation of plasmid DNA from small batch cultures of Escherichia coli. Norgen’s magnetic beads bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. The plasmid DNA is preferentially purified from other cellular components such as genomic DNA and RNA. The purified plasmids are fully digestible with all restriction enzymes tested, and are completely compatible with real-time PCR and NGS.
Norgen's Plasmid MiniPrep Kit (Magnetic Bead System) is also available in a 96-well (HT) format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.
Plasmid MaxiPrep Kit
This kit is designed for the rapid spin column preparation of plasmid DNA from up to 100 mL of Escherichia coli cultures. The kit allows for the isolation of plasmid DNA with final endotoxin levels of 0.1 EU/µg of DNA or less. The kit is able to purify plasmids up to 13,000 bp in size, and typical yields from a 100 mL culture for a high copy number plasmid are between 0.4 and 1.0 mg. The purified DNA is fully digestible with all restriction enzymes tested, and is completely compatible with manual or automated sequencing to achieve 95-100% accuracy.
Details
Supporting Data
Kit Specifications
|
|
Column Binding Capacity |
25 µg
|
Size of Plasmids Purified |
Up to 13,000 bp
|
Average Yield from 1.5 mL of Culture |
Up to 20 µg
|
Time to Complete 10 Purifications |
30 minutes
|
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the of shipment. The RNase should be stored at -20°C upon arrival. The Resuspension Solution AZ should be stored at 4°C upon addition of RNase enzyme.
Component | Cat. 13300 (50 preps) | Cat. 46400 (250 preps) | Cat. 46500 (4 preps) | Cat. 46600 (20 preps) | Cat. 60300 (50 preps) | Cat. 63000 (192 preps) |
---|---|---|---|---|---|---|
Resuspension Solution AZ | 12 mL | 60 mL | 20 mL | 100 mL | 12 mL | 2 x 20 mL |
Lysis Buffer N | 40 mL | 80 mL | 40 mL | 2 x 80 mL | 40 mL | 2 x 40 mL |
Buffer TN | 20 mL | 130 mL | 55 mL | 2 x 130 mL | 20 mL | 1 x 55 mL 1 x 20 mL |
Wash Solution E | 12 mL | 2 x 18 mL | - | - | - | - |
Elution Buffer K | 8 mL | 30 mL | - | - | 8 mL | 2 x 8 mL |
Wash Solution J | - | - | 25 mL | 3 x 25 mL | - | - |
Elution Buffer J | - | - | 24 mL | 120 mL | - | - |
RNase A | 1 vial | 1 vial | 1 vial | 1 vial | 1 vial | 1 vial |
Magnetic Bead Suspension | - | - | - | - | 1 x 1.1 mL | 4 x 1.1 mL |
Spin Columns | 50 | 250 | - | - | - | - |
Collection Tubes | 50 | 250 | - | - | - | - |
DNA Maxi Spin Columns with Collection Tubes (Clear ring in column) | - | - | 4 | 20 | - | - |
Maxi Spin Filter Columns with Collection Tubes (Grey ring in column) | - | - | 4 | 20 | - | - |
96-Well Plate | - | - | - | - | - | 2 |
Elution Tubes (1.7 mL) | 50 | 250 | - | - | 50 | - |
Elution Tubes (50 mL) | - | - | 4 | 20 | ||
96-Well Elution Plate | - | - | - | - | - | 2 |
Adhesive Tape | - | - | - | - | - | 2 |
Product Insert | 1 | 1 | 1 | 1 | 1 | 1 |
Documentation
(46400) Plasmid MiniPrep Kit - (250 prep)
(46500) Plasmid DNA MaxiPrep Kit - Protocol (4 prep)
(46600) Plasmid DNA MaxiPrep Kit - Protocol (20 prep)
(60300) Plasmid MiniPrep Kit (Magnetic Bead System) - Protocol (50 prep)
(63000) Plasmid MiniPrep 96-Well Kit (High Throughput Magnetic Bead System) - Protocol (2 x 96-well)
FAQs
MiniPrep, MiniPrep Magnetic Bead System, High Throughput MiniPrep Magnetic Bead System, MaxiPrep
Poor DNA recovery could be due to one or more of the following:
- Plasmid did not propagate.
Ensure that the appropriate growth medium, supplements, and antibiotics were used for the host cell and plasmid of interest.
- Inoculum cell culture was old.
Old bacterial cells are a poor source of plasmid DNA. Bacterial cell inoculum should be prepared from fresh single colonies, grown in a test tube overnight, and immediately used for inoculum preparation. Prolonged incubation or storage of culture in the fridge almost guarantees poor results.
- Insufficient lysis of cells.
The Lysis Buffer N may have formed precipitates. Warm and mix gently before use.
- Cell resuspension was incomplete.
Pelleted cells should be completely resuspended in the Resuspension Solution AZ. Do not add Lysis Buffer N until a homogeneous suspension is obtained.
- Elution Buffer was not placed directly over the column’s membrane.
It is important that the Elution Buffer be placed directly over the column’s membrane to ensure uniform passing of the buffer through the column. Do not pipette the Elution Buffer K onto the side of the column.
- Proper Elution Buffer was not used.
The provided Elution Buffer has been optimized for high elution recoveries for respective plasmid DNA isolation kits. If water is used, ensure that the pH is between 7 and 8.
If the DNA does not perform well in downstream applications, it may be due to one or more of the following:
- Bound DNA was not washed properly.
Traces of salt from the binding step may remain in the sample if the column is not washed with Wash Buffer E (for miniprep) or Wash Buffer J (for maxiprep) / magnetic beads are not washed with 70% ethanol (freshly prepared). Salt may interfere with downstream applications and thus must be washed from the magnetic beads.
- Dry spin not performed.
The dry spin must be performed after the wash step in order to remove all traces of ethanol.
- The appropriate amount of ethanol was not added to the Wash Solution E / Wash Solution J.
The Wash Solution E (miniprep) / Wash Solution J (maxiprep) has been specifically designed to contain the appropriate amount of components. Ensure that the Wash Solution was prepared using the correct amount of ethanol.
- A different elution buffer was used.
If a different elution buffer other than the one provided in the kit was used, the buffer should be checked for any components that may interfere with the application. Common components that are known to interfere are high salts (including EDTA), detergents, and other denaturants. Check the compatibility of your elution buffer with the intended use.
Citations
MiniPrep (13300, 46400)
Title | Differences in Gene Expression of Pear Selections Showing Leaf Curling or Leaf Reddening Symptoms Due to Pear Decline Phytoplasma |
Journal | Plants (Basel). 2022. |
Authors | Mina Kaviani, Paul H Goodwin, David M Hunter |
Title | Evaluation of aromatic and polycyclic hydrocarbon degrading abilities of selected bacterial isolates |
Journal | Journal of Microbiology and Biotechnology Research. 2017 |
Authors | Thenmozhi. R, D.Praveenkumar, E.Priya, A. Nagasathy, N.Thajuddin |
Title | Antimicrobial Resistance Profiles of Listeria monocytogenes and Listeria innocua Isolated from Ready-to-Eat Products of Animal Origin in Spain |
Journal | Foodborne Pathogens and Disease. 2017 |
Authors | Escolar C, Gómez D, Rota García MD, Conchello P, Herrera A. |
Title | Plasmid profile of Staphylococcus aureus from orthopaedic patients in Ahmadu Bello University Teaching Hospital Zaria, Nigeria |
Journal | African Journal of Microbiology Research. 2015. |
Authors | Obajuluwa, A. F., Olayinka, B. O., Adeshina, G. O., Onaolapo, J. A. and Afolabi-Balogun, N. B. |
MaxiPrep (46500, 46600)
Title | Role of Endonuclease G in Exogenous DNA Stability in HeLa Cells |
Journal | International Journal of Biotechnology and Bioengineering. 2016. |
Authors | Vanja Misic, Mohamed El-Mogy, Yousef Haj-Ahmad |