Soil DNA Isolation Kit (Magnetic Bead System)
For isolating genomic DNA from soil samples
For research use only and NOT intended for in vitro diagnostics.
Soil DNA Isolation Kit (Magnetic Bead System)
For isolating genomic DNA from soil samples
Overview
- Fast and easy processing using a magnetic bead system
- Robust lysis system (chemical lysis combined with a mechanical homogenization)
- Isolate high quality genomic DNA
- High yields - Consistent, high yields of inhibitor-free DNA up to 50 kb plus
- Isolate sequencing quality total DNA from a variety of microorganisms including bacteria, fungi and algae
- Also available in a 96-well format that can be integrated with a robotic automation system
Norgen’s Soil DNA Isolation Kit (Magnetic Bead System) provides a fast and reproducible method for isolating genomic DNA from soil samples. All types of soil samples can be processed with this kit, including common soil samples and difficult soil samples with high humic acid content such as compost and manure. The kit removes all traces of humic acid using the provided the OSR (Organic Substance Removal) Solution. Total genomic DNA can be isolated and purified from all the various microorganisms found in soil, such as bacteria, fungi and algae. The purified DNA is of the highest quality and is fully compatible with downstream PCR applications, as all humic acid substances and PCR inhibitors are removed during the isolation.
Norgen's Soil DNA Isolation Kit (Magnetic Bead System) is also available in a 96-well format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.
Details
Supporting Data
Kit Specifications
|
|
Maximum Soil Input
|
0.25 g
|
Type of Soil Processed
|
All types of soil
|
Average Yield from 0.25 mL of Soil*
|
1 - 5 μg
|
Average Purity (OD260/280) |
1.7 - 1.8
|
Time to Complete Purifications |
40 minutes (Cat. 58100)
50 minutes (Cat. 62800) |
* Average DNA yields will vary depending upon soil sample types
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
Component | Cat. 58100 (50 preps) | Cat. 62800 (2 x 96-well) |
---|---|---|
Lysis Buffer G | 100 mL | 1 x 100 mL 1 x 45 mL |
Lysis Additive A | 6 mL | 25 mL |
Binding Buffer I | 7 mL | 25 mL |
OSR Solution | 3 mL | 12 mL |
Binding Buffer B | 2 x 12 mL | 85 mL |
Magnetic Bead Suspension | 2 x 1.1 mL | 8.5 mL |
Buffer SK | 30 mL |
1 x 40 mL 1 x 60 mL |
Elution Buffer B | 8 mL | 15 mL |
Elution Tubes (1.7 mL) | 50 | - |
Bead Tubes | 50 | 196 |
96-Well Plate | - | 2 |
96-Well Elution Plate | - | 2 |
Adhesive Tape | - | 2 |
Product Insert | 1 | 1 |
Documentation
FAQs
Magnetic Bead System, High Throughput Magnetic Bead System
If the magnetic beads were accidently pipetted up with the supernatant, the pipette tip was placed too close to the magnetic beads while pipetting. Simply return the magnetic beads and the supernatant back into the sample well. Mix well, and place the plate back onto the magnetic separation plate for the specified time. Carefully remove the supernatant without touching the magnetic beads
A low genomic DNA yield may be caused by the following:
- Incomplete lysis of cells.
Ensure that Lysis Additive A is added. Also, incubation at 65ºC may result in increased yields.
- Amount of magnetic beads added was not sufficient.
Ensure that the magnetic bead suspension is mixed well prior to use to avoid any inconsistency in DNA isolation.
- DNA concentration in the soil sample being used is low.
Some soil types contain very little target DNA. Incubation at 65ºC may result in increased yields.
If the DNA does not perform well in downstream applications, it may be due to one or more of the following:
- Eluted DNA sample is brown.
The elution contains high humic acids. Ensure that the OSR Solution was added to the clean lysate.
- DNA was not washed with the provided Buffer SK.
Traces of humic acids or salt from the binding step may remain in the sample if the magnetic beads are not washed with the provided Buffer SK. Humic acids and salt may interfere with downstream applications and thus must be washed from the magnetic beads.
- Ethanol carryover.
Ensure that the drying step after the 70% ethanol wash steps is performed in order to remove traces of ethanol prior to elution. Ethanol is known to interfere with many downstream applications.
Ran can get co-eluted with DNA. Carry out a digestion with RNase A on the elution if the RNA present will interfere with downstream applications. Refer to manufacturer’s instructions regarding amount of enzyme to use, optimal incubation time and temperature.