Urine Cell-Free Circulating and Viral Nucleic Acid Purification Kits
For isolating total circulating nucleic acids (DNA and RNA) from various urine inputs ranges
For research use only and NOT intended for in vitro diagnostics.
Urine Cell-Free Circulating and Viral Nucleic Acid Purification Kits
For isolating total circulating nucleic acids (DNA and RNA) from various urine inputs ranges
Overview
- Isolate all sizes of circulating DNA, circulating and exosomal RNA, including microRNA, viral DNA/RNA in one elution
- Versatile urine input ranges
- No phenol extractions nor carrier RNA
- Bind and elute all RNA irrespective of size or GC content, without bias
- Concentrate circulating DNA, circulating RNA and exosomal RNA, viral DNA, viral RNA into a flexible elution volumes
- Compatible with fresh, frozen or preserved urine sample
- Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services
- Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
These kits provide a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating DNA, circulating RNA including exosomal RNA as well as viral DNA/RNA from fresh, frozen or preserved urine samples. Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix. These kits are designed to isolate all sizes of cfc-DNA and cfc-RNA, including microRNA, as well as all sizes of exosomal RNA. Norgen’s Urine Cell-Free Circulating and Viral Nucleic Acid Purification Kits provide a clear advantage over other available kits in that they do not require phenol/chloroform or any protease treatments. Moreover, the kits allow the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The purified nucleic Acid is of the highest integrity, and can be used in a any downstream applications including PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, methylation-sensitive PCR and Southern Blot analysis, Northern blotting, RNase protection and primer extension, expression array assays, and NGS.
Background
Recent evidence indicates that cell-free circulating (cfc) nucleic acids such as DNA and RNA, including exosomal RNA, in urine contains valuable information for the discovery of biomarkers that can help with early detection of certain cancer types, for monitoring the disease status as well as for the detection fetal DNA. Moreover, urine has been widely used for the detection of viral infections, including HIV, HCV and HBV.The advantage for using urine as a source for cancer biomarkers is that it can be acquired in large quantities without using invasive procedures, and repeated sampling from the same individual is applicable, which facilitates longitudinal studies. There are many advantages favouring the use of urinary nucleic acids for cancer biomarker discovery over blood, tissue samples or other bodily fluids, including: (1) urine is non-infectious for HIV and less infectious for many other pathogens; (2) the profile of urinary nucleic acids is similar to that in urine or serum; (3) nucleic acid purification from urine is technically much easier because of its low protein concentration (1000-fold lower than blood).
Urine Cell-Free Circulating and Viral Nucleic Acid Purification Mini Kit
For sample input volumes ranging from 250 µL to 2 mL.
Urine Cell-Free Circulating and Viral Nucleic Acid Purification Midi Kit
For sample input volumes ranging from 2 mL - 10 mL.
Urine Cell-Free Circulating and Viral Nucleic Acid Purification Maxi Kit
For sample input volumes ranging from 10 mL - 30 mL.
Details
Supporting Data
Kit Specifications
|
|
Minimum Urine Input
|
250 μL
|
Maximum Urine Input
|
2 mL
|
Size of Nucleic Acid Purified |
All sizes, including miRNA and
small RNA (< 200 nt) |
Elution Volume |
50-100 μL
|
Time to Complete 10 Purifications |
25-30 minutes
|
Average Yields |
Variable depending on specimen
|
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. Theses kits are stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
Component | Cat. 59900 (50 preps) | Cat. 60000 (20 preps) | Cat. 60100 (10 preps) |
---|---|---|---|
Binding Solution K | 25 mL | 75 mL | 1 x 75 mL 1 x 25 mL |
Lysis Buffer A | 30 mL | 20 mL | 20 mL |
Wash Solution A | 18 mL | 18 mL | 18 mL |
Elution Buffer F | 6 mL | 6 mL | 6 mL |
Mini Spin Columns | 50 | 20 | 10 |
Midi Spin Columns | - | 20 | - |
Maxi Spin Columns | - | - | 10 |
Collection Tubes | 50 | 20 | 10 |
Elution Tubes (1.7 mL) | 50 | 20 | 10 |
Product Insert | 1 | 1 | 1 |